Molecular Plant Biology Volume 2

Molecular Plant Biology Volume 2

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Volume Two focuses on experimental approaches for studies on gene expression, gene product analysis, with the final section devoted to emerging technologies. Topics covered include a range of techniques for transcript analysis, including In situ Hybridization and DNA microarrays. DNA-protein interaction methods are also covered in detail. Inducible gene expression in plants as well as expression and analysis of recombinant proteins, and analysis of protein import into chloroplasts are covered as well as techniques for fractionation of plant tissue for biochemical analyses and the study of protein-protein interactions with the yeast two-hybrid system. A range of approaches for using antibodies as tools are also described including the use of antibody phage display libraries. The final section on emerging technologies describes methodologies for calcium imaging and for the spatial and temporal analysis of reporter genes such as luciferase and green fluorescent protein. The final area covers a range of experimental procedures for moss, which is emerging as a new model organism.

    Binding

    Paperback

    Creator

    Philip M. Gilmartin

    Creator

    Chris Bowler

    EAN

    9780199638185

    Edition

    1st

    ISBN

    0199638187

    Label

    Oxford University Press

    Manufacturer

    Oxford University Press

    NumberOfPages

    368

    ProductGroup

    Book

    ProductTypeName

    ABIS_BOOK

    PublicationDate

    2002-06-15

    Publisher

    Oxford University Press

    Studio

    Oxford University Press

    Molecular Plant Biology Volume 2

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